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The suspension was incubated for 1 h at room temperature, before the membranes were isolated by centrifugation at 40,000 g for 30 min
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For type 2 diabetes (Ozempic), the same 0.25 mg starting dose applies, but the maximum dose is 2.0 mg weekly
The cells were treated with these cocktails for 3 days, with variations in CHIR concentration and the presence of Ly or PD to optimize DE induction efficiency [1]
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